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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Canine Fibrinogen ELISA Kit (Sandwich ELISA) - LS-F36790
LS-F36790 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Dog Fibrinogen in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Fibrinogen as low as 37.5 picograms per milliliter.
Catalog
Size
Price
LS-F36790-1
1 plate
$870

Popular Fibrinogen Elisa Kits

Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Pig
Range: 15.63-1000 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Plasma
Type: Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Bovine
Range: 39-2500 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Human
Range: 3.125-800 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 3.125-800 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Rabbit
Range: 0.5-500 ng/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Plasma

Product Description

LS-F36790 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Dog Fibrinogen in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of Fibrinogen as low as 37.5 picograms per milliliter.
About Fibrinogen
Fibrinogen (factor I) is a glycoprotein in vertebrates that helps in the formation of blood clots. It consists of a linear array of three nodules held together by a very thin thread which is estimated to have a diameter between 8 and 15 Angstrom (Å). The two end nodules are alike but the center one is slightly smaller. Measurements of shadow lengths indicate that nodule diameters are in the range 50 to 70 Angstrom. The length of the dried molecule is 475 ± 25 Angstrom.

Specifications

Type
Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target
Fibrinogen
Reactivity
Canine
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Format
96-Well Strip Plate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
62.5 - 4000 pg/ml
Sensitivity
37.5
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
  • Adhesive Plate Sealers
  • Coated 96-well Strip Plate
  • Detection Antibody Diluent
  • Biotinylated Detection Antibody (100x)
  • HRP-Streptavidin Conjugate (100x)
  • HRP-Streptavidin Conjugate Diluent
  • Sample Diluent
  • TMB Substrate
  • Stop Solution
  • Wash Buffer (25x)
  • Standard (Lyophilized)
Background
This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fbg antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fbg antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fbg amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Fbg can be calculated.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.

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Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Sandwich ELISA Platform Overview
Sandwich ELISA Platform Overview

Requested From: United States
Date Requested: 8/14/2018

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