Competitive EIA (enzyme immunoassay) kit
Intended Sample Types
Plasma, Serum, Tissue Homogenates
96-Well Strip Plate
Colorimetric - 450nm (TMB)
15.6 - 1000 pg/ml
Intra-Assay: CV<8% Inter-Assay: CV<10%
Store at 4°C. Stable for 6 months.
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
- Coated 96-well Strip Plate
- Detection Antibody Diluent
- Biotinylated Detection Antibody (100x)
- HRP-Streptavidin Conjugate (100x)
- HRP Streptavidin Conjugate Diluent
- Sample Diluent
- Standard (Lyophilized)
- TMB Substrate
- Stop Solution
- Wash Buffer (25x)
- Adhesive Plate Sealers
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Estrogen. During the reaction, Estrogen in the sample or standard competes with a fixed amount of Estrogen on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Estrogen. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Estrogen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
For research use only.