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Catalog Number Size Price
LS-H8049-1 1 mg $55 
LS-H8049-5 5 mg $165 
LS-H8049-10 10 mg $265 
Biochemical - GW 4869 (hydrochloride hydrate) Structure

GW 4869 (hydrochloride hydrate) (CAS 6823-69-4) - LS-H8049

GW 4869 (hydrochloride hydrate) (CAS 6823-69-4) - LS-H8049

Available for shipment within the USA only
Description:
Neutral sphingomyelinases mediate the release of ceramide from sphingomyelin in cellular membranes and can be activated by certain stresses. Ceramide can act as a signaling molecule in its own right, or it can be further processed to generate sphingosine (and sphingosine-1-phosphate). GW 4869 is a cell-permeable, non-competitive inhibitor of neutral sphingomyelinases (IC50 = 1 µM), but does not affect acid sphingomyelinase activity. It inhibits TNF-Alpha-mediated sphingomyelin hydrolysis (100% inhibition at 20 µM) 1 and TNF-Alpha-induced cell death in MCF-7 cells without interfering with activation of NF-KB or glutathione depletion in response to TNF. GW 4869 also reduces the inhibitory effect of oxidized phospholipid products on lipopolysaccharides-mediated induction of interleukin-8 in human aortic endothelial cells 3 and prevents hypoxia-induced pulmonary vasoconstriction in rats in vivo.
Price
Catalog Number
$55 
LS-H8049-1

Available for shipment within the USA only
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
Neutral sphingomyelinases mediate the release of ceramide from sphingomyelin in cellular membranes and can be activated by certain stresses. Ceramide can act as a signaling molecule in its own right, or it can be further processed to generate sphingosine (and sphingosine-1-phosphate). GW 4869 is a cell-permeable, non-competitive inhibitor of neutral sphingomyelinases (IC50 = 1 µM), but does not affect acid sphingomyelinase activity. It inhibits TNF-Alpha-mediated sphingomyelin hydrolysis (100% inhibition at 20 µM) 1 and TNF-Alpha-induced cell death in MCF-7 cells without interfering with activation of NF-KB or glutathione depletion in response to TNF. GW 4869 also reduces the inhibitory effect of oxidized phospholipid products on lipopolysaccharides-mediated induction of interleukin-8 in human aortic endothelial cells 3 and prevents hypoxia-induced pulmonary vasoconstriction in rats in vivo.

Specifications

Conjugations
Unconjugated
CAS Number
6823-69-4
Biological Function
Inhibitor of neutral sphingomyelinase.
Purity
Greater than 90%
Formal Name
3,3'-(1,4-phenylene)bis[N-[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-dihydrochloride-2-propenamide
Classification
Biochemical, Chemical
Molecular Formula
C30H28N6O2• 2HCl [XH2O]
Molecular Weight
577.5
Formulation
a crystalline solid
λmax
346 nm
SMILES
O=C(NC1=CC=C(C2=NCCN2)C=C1)/C=C/C3=CC=C(/C=C/C(NC4=CC=C(C5=NCCN5)C=C4)=O)C=C3.Cl.Cl.O
InChI Key
DKFSVQGRETYFBG-ZAKYHKHWSA-N
Storage
Store at -20°C for up to 2 years.
Documents
Usage Information
GW 4869 (hydrochloride hydrate) is supplied as a crystalline solid. A stock solution may be made by dissolving the GW 4869 (hydrochloride hydrate) in an organic solvent purged with an inert gas. GW 4869 (hydrochloride hydrate) is soluble in DMSO. The solubility of GW 4869 (hydrochloride hydrate) in DMSO is approximately 0.2 mg/ml. If aqueous stock solutions are required for biological experiments, they can best be prepared by diluting the organic solvent into aqueous buffers or isotonic saline. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
Restrictions
For research use only. Available for shipment within the USA only
Guarantee
This Biochemical carries the LSBio 100% Guarantee

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Biochemical - GW 4869 (hydrochloride hydrate) Structure
GW 4869 (hydrochloride hydrate) Structure

Biochemical - GW 4869 (hydrochloride hydrate) Structure
GW 4869 (hydrochloride hydrate) Structure

Biochemical - GW 4869 (hydrochloride hydrate) Structure
GW 4869 (hydrochloride hydrate) Structure

Biochemical - GW 4869 (hydrochloride hydrate) Structure
GW 4869 (hydrochloride hydrate) Structure

Requested From: United States
Date Requested: 2/28/2020