Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The cysteinyl-leukotrienes (CysLTs), LTC4 and LTD4, are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. Their biological effects are transduced by a pair of G protein-coupled receptors, CysLT1 and CysLT2. Zafirlukast is a potent, selective cysteinyl-leukotrienes 1 (CysLT1) receptor antagonist sold under the trade name Accolate for the treatment of asthma as well as for the symptoms associated with allergic rhinitis. It binds to the human CysLT1 and CysLT2 receptors with IC50 values of approximately 5 and 7,400 nM, respectively.
Zafirlukast is supplied as a crystalline solid. A stock solution may be made by dissolving the zafirlukast in an organic solvent purged with an inert gas. Zafirlukast is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of zafirlukast in these solvents is approximately 0.25, 20, and 30 mg/ml, respectively. Zafirlukast is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, zafirlukast should first be dissolved in DMF and then diluted with the aqueous buffer of choice. Zafirlukast has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.