Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Alpha / beta-Hydrolase domain-containing protein 6 (ABHD6) is a serine hydrolase that is highly expressed in mammalian brain. This enzyme catalyzes the hydrolysis of the endocannabinoid 2-arachidonoyl glycerol (2-AG). WWL70 is a selective inhibitor of ABHD6 with an IC50 value of 70 nM.
WWL70 is supplied as a crystalline solid. A stock solution may be made by dissolving the WWL70 in an organic solvent purged with an inert gas. WWL70 is soluble in organic solvents such as DMSO and dimethyl formamide (DMF). The solubility of WWL70 in these solvents is approximately 1 mg/ml. WWL70 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, WWL70 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. WWL70 has a solubility of approximately 0.2 mg/mlin a 1:3 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Blankman, J.L., Simon, G.M., and Cravatt, B.F. A comprehensive profile of brain enzymes that hydrolyze the endocannabinoid 2-arachidonoylglycerol. Chemistry & Biology 14, 1347-1356 (2007).
Li, W., Blankman, J.L., and Cravatt, B.F. A functional proteomic strategy to discover inhibitors for uncharacterized hydrolases. Journal of American Chemical Society 129, 9594-9595 (2007).