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SPD-304 (CAS 869998-49-2) LS-H15

TNF alpha inhibitor
LS-H15-500 / 500 µg / $175
LS-H15-1000 / 1000 µg / $195
LS-H15-5000 / 5000 µg / $285
LS-H15-10000 / 10000 µg / $385
USA Shipment Only
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Description: Tumor necrosis factor alpha (TNF alpha) is a primary mediator of many inflammatory conditions including rheumatoid arthritis, toxic shock, and sepsis. TNF alpha functions as a trimer and promotes receptor trimerization to activate proinflammatory and/or apoptotic signalling pathways. SPD-304 is an inhibitor of tumor necrosis factor alpha (TNF alpha). that prevents binding to the TNF Receptor 1 (TNFR1) with an IC50 of 22 µM. It binds to the biologically active TNF alpha trimer and promotes accelerated displacement of a single subunit to rapidly inactivate the cytokine. In a cell based assay, SPD-304 inhibited TNF alpha -mediated stimulation of IKB degradation with an IC50 of 4.6 µM. H15_ChemicalStructureImage.png
6,7-dimethyl-3-[[methyl[2-[methyl[[1-[3-(trifluoromethyl)phenyl]-1H-indol-3-yl]methyl]amino]ethyl]amino]methyl]-4H-1-benzopyran-4-one
 
biochemical, chemical, inhibitor
LS-H15
869998-49-2
C32H32F3N3O2
≥98%
A crystalline solid
 
CN(CCN(C)Cc1cn(c2cccc(c2)C(F)(F)F)c2ccccc12)Cc1coc2cc(C)c(C)cc2c1=O
InChI=1S/C32H32F3N3O2/c1-21-14-28-30(15-22(21)2)40-20-24(31(28)39)18-37(4)13-12-36(3)17-23-19-38(29-11-6-5-10-27(23)29)26-9-7-8-25(16-26)32(33,34)35/h5-11,14-16,19-20H,12-13,17-18H2,1-4H3
JZNXLPPJRFFECJ-UHFFFAOYSA-N
Shipped Ambient, store at -20°C, ≥ 2 years shelf life.
This product is for research use only. Not for administration to humans, or for human or veterinary diagnostic or therapeutic use.
Data Sheet DataSheet    SDS SDS
Related Products: TNF Alpha related products

Usage Information

SPD-304 is supplied as a crystalline solid. A stock solution may be made by dissolving the SPD-304 in an organic solvent purged with an inert gas. SPD-304 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of SPD-304 in these solvents is approximately 1 mg/ml in ethanol and DMF and approximately 10 mg/ml in DMSO. SPD-304 is sparingly soluble in aqueous buffers. If aqueous stock solutions are required for biological experiments, they can best be prepared by diluting the organic solvent into aqueous buffers or isotonic saline. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.

References:
  1. Beutler, B., Greenwald, D., Hulmes, J.D., et al. Identity of tumor necrosis factor a/cachectin and tumor necrosis factor b/lymphotoxin on hematopoietic growth factor production and neutrophil adhesion molecule expression by cultured human endothelial cells. J. Immunol. 138, 4298-4302 (1987).
  2. Tracey, K.J., Beutler, B, B., Lowry, S.F., et al. Shock and tissue injury induced by recombinant human cachetin. Science 234, 470-474 (1986).
  3. Bidgood, M.J., Jamal, O.S., Cunningham, A.M., et al. Type IIA secretory phospholipase A2 up-regulates cyclooxygenase-2 and amplifies cytokine-mediated prostaglandin production in human rheumatoid synoviocytes. J. Immunol. 165, 2790-2797 (2000).
  4. Thornberry, N.A. and Lazebnik, Y. Caspases: Enemies within. Science 281, 1312-1316 (1998).
  5. Wallach, D., Varfolomeev, E.E., Malinin, N.L., et al. Tumor necrosis factor receptor and Fas signaling mechanisms. Ann. Rev. Immunol. 17, 133-167 (1999).

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