Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: SIRT1/2 inhibitor IV is a cell-permeable inhibitor of SIRT1 (IC50 = 56 µM) and SIRT2 (IC50 = 59 µM), blocking NAD+-dependent deacetylase activity in a substrate competitive manner. It less effectively inhibits SIRT5 (IC50 >300 µM) and has no effect on class I and II histone deacetylases. By inhibiting SIRT1, SIRT1/2 inhibitor IV sensitizes H460 lung cancer cells to etoposide and paclitaxel and enhances etoposide-induced G2 arrest. It also blocks a SIRT1-dependent hypoxic response in vivo, suppressing HIF-1 alpha protein accumulation as well as EPO and VEGF expression in HepG2 tumors in mice.
SIRT1/2 inhibitor IV is supplied as a crystalline solid. A stock solution may be made by dissolving the SIRT1/2 inhibitor IV in the solvent of choice. SIRT1/2 inhibitor IV is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of SIRT1/2 inhibitor IV in these solvents is approximately 0.33, 20, and 16 mg/ml, respectively. SIRT1/2 inhibitor IV is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, SIRT1/2 inhibitor IV should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. SIRT1/2 inhibitor IV has a solubility of approximately 0.14 mg/ml in a 1:6 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Heltweg, B., Gatbonton, T., Schuler, A.D., et al. Antitumor activity of a small-molecule inhibitor of human silent information regulator 2 enzymes. Cancer Res. 66, 4368-4377 (2006).
Laemmle, A., Lechleiter, A., Roh, V., et al. Inhibition of SIRT1 impairs the accumulation and transcriptional activity of HIF-1a protein under hypoxic conditions. PLoS One 7(3), (2012).