Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Philanthotoxin 74 (PhTx-74) is a synthetic analog of the naturally-occurring wasp venom toxin philanthotoxin-4,3,3. It is a subtype-selective AMPA receptor antagonist that inhibits homomeric GluR1 and GluR3 as well as heteromeric GluR1/2 receptors (80-100% of glutamate-evoked currents inhibited at 100 µM). It exhibits minimal to no effect on monomeric GluR2 or heteromeric GluR2/3 receptors at concentrations up to 500 µM. PhTx-74 inhibits the GluR5(Q) receptor with a Ki value of 0.29 µM.
PhTx-74 (hydrochloride) is supplied as a white to off-white solid. A stock solution may be made by dissolving the PhTx-74 (hydrochloride) in the solvent of choice. PhTx-74 (hydrochloride) is soluble in the organic solvent DMSO. It is also soluble in water. The solubility of PhTx-74 (hydrochloride) in DMSO and water is approximately 100 mM. We do not recommend storing the aqueous solution for more than one day.
Nilsen, A. and England, P.M. A subtype-selective, use-dependent inhibitor of native AMPA receptors. J. Am. Chem. Soc. 129(16), 4902-4903 (2007).
Kromann, H., Krikstolaityte, S., Andersen, A.J., et al. Solid-phase synthesis of polyamine toxin analogues: Potent and selective antagonists of Ca2+-permeable AMPA receptors. J. Med. Chem. 45(26), 5745-5754 (2002).