Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Pemirolast is a histamine H1 receptor antagonist and a mast cell stabilizer that prevents the release of histamine. It also blocks the release of substance P, neurokinin A, and calcitonin gene-related peptide from sensory nerves. Pemirolast has been used for the treatment of allergic conjunctivitis.
9-methyl-3-(2H-tetrazol-5-yl)-4H-pyrido[1,2-a]pyrimidin-4-one, monopotassium salt
Pemirolast (potassium salt) is supplied as a crystalline solid. A stock solution may be made by dissolving the pemirolast (potassium salt) in the solvent of choice. Pemirolast (potassium salt) is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of pemirolast (potassium salt) in these solvents is approximately 1 and 0.1 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of pemirolast (potassium salt) can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of pemirolast (potassium salt) in PBS, pH 7.2, is approximately 10 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Yanagihara, Y., Kasai, H., Matsui, S., et al. Immunopharmacological studies on TBX, a new antiallergic drug (3). Inhibitory effects on histamine release from lung fragments and bronchoconstriction in guinea pigs. Jpn. J. Pharmacol. 51(1), 83-92 (1989).
Tatsushima, Y., Egashira, N., Kawashiri, T., et al. Involvement of substance P in peripheral neuropathy induced by paclitaxel but not oxaliplatin. J. Pharmacol. Exp. Ther. 337(1), 226-235 (2011).
Minami, K., Hossen, M.A., and Kamei, C. Increasing effect by simultaneous use of levocabastine and pemirolast on experimental allergic conjunctivitis in rats. Biol. Pharm. Bull. 28(3), 473-476 (2005).