Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Leukotriene B4 (LTB4) is a dihydroxy fatty acid derived from the 5-lipoxygenase pathway of arachidonic acid metabolism and is an important mediator of the inflammatory process. The benzophenone dicarboxylic acid derivative LY223982 is a potent BLT1 receptor antagonist. It inhibits the specific binding of radiolabeled-LTB4 to isolated human neutrophils with an IC50 value of 13.2 nM. LY223982 inhibits the leukotriene B4 (LTB4)-induced aggregation of guinea pig and human neutrophils with IC50 values of 74 and 100 nM, respectively. However, concentrations of LY223982 up to 10 µM do not inhibit binding of LTB4 to human BLT1 or BLT2 expressed in CHO cells.
LY223982 is supplied as a crystalline solid. A stock solution may be made by dissolving the LY223982 in an organic solvent purged with an inert gas. LY223982 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of LY223982 in ethanol is approximately 0.1 mg/ml and approximately 20 mg/ml in DMSO and DMF. LY223982 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, LY223982 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. LY223982 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Gapinski, D.M., Mallett, B.E., Froelich, L.L., et al. Benzophenone dicarboxylic acid antagonists of leukotriene B4.
Structure-activity relationships of the lipophilic side chain. J. Med. Chem. 33, 2807-2813 (1990). 2. Jackson, W.T., Boyd, R.J., Froelich, L.L., et al. Specific inhibition of leukotriene B4-induced neutrophil activation by LY223982. J. Pharmacol. Exp. Ther. 263(3), 1009-1014 (1992).