Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase is the rate-limiting enzyme in the cholesterol biosynthetic pathway and the target of the `statin' class of cholesterol-lowering drugs. Lovastatin is a HMG-CoA reductase inhibitor that was initially isolated from Aspergillus terreus. Marketed as Mevinolinâ„¢, Mevacorâ„¢, and under several other trade names, lovastatin was the first HMG-CoA reductase inhibitor to be used in the treatment of hypercholesterolemia. It is a competitive inhibitor of HMG-CoA with a Ki value of 0.6 nM for the open ring hydroxy acid form of the molecule. During a three week study in dogs, a dose of 8 mg/kg per day resulted in a 29% reduction in plasma cholesterol. Lovastatin also suppresses TNF-induced NF- kappa B activation (IC50 ~ 15 µM), which potentiates apoptosis in human myeloid leukemia cells and thus, may be useful in treating cancer.
2-methyl-1S,2,3R,7S,8S,8aR-hexahydro-3,7-dimethyl-8-[2-[(2R,4R)-tetrahydro-4-hydroxy-6-oxo-2H-pyran-2-yl]ethyl]-1-naphthalenyl ester, butanoic acid, monosodium salt
Lovastatin (sodium salt) is supplied as a crystalline solid. A stock solution may be made by dissolving the lovastatin (sodium salt) in an organic solvent purged with an inert gas. Lovastatin (sodium salt) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of lovastatin (sodium salt) in these solvents is approximately 10 mg/ml. If aqueous stock solutions are required for biological experiments, they can best be prepared by diluting the organic solvent into aqueous buffers or isotonic saline. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
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Alberts, A.W., Chen, J., Kuron, G., et al. Proc. Natl. Acad. Sci. USA 77(7), 3957-3961 (1980).