Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Loratadine is a non-sedating antihistamine that acts as a selective inverse agonist of peripheral histamine H1 receptors (Ki = 35 nM). It has been shown to inhibit the release of leukotriene C4 (IC50 = 8 µM) and histamine (IC50 = 11 µM) from rodent mast cells and to inhibit allergic bronchospasm in guinea pigs with an ED50 value of 0.40 mg/kg.
Loratadine is supplied as a crystalline solid. A stock solution may be made by dissolving the loratadine in the solvent of choice. Loratadine is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of loratadine in ethanol and DMF is approximately 30 mg/ml and approximately 25 mg/ml in DMSO. Loratadine is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, loratadine should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. Loratadine has a solubility of approximately 0.25 mg/ml in a 1:3 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Ahn, H.S. and Barnett, A. Selective displacement of [3H]mepyramine from peripheral vs. central nervous system receptors by loratadine, a non-sedating antihistamine. Eur. J. Pharmacol. 127(1-2), 153-155 (1986).
Kay, G.G. and Harris, A.G. Loratadine: A non-sedating antihistamine. Review of its effects on cognition, psychomotor performance, mood and sedation. Clin. Exp. Allergy 29, 147-150 (1999).
Barnett, A., Iorio, L.C., Kreutner, W., et al. Evaluation of the CNS properties of SCH 29851, a potential non-sedating antihistamine. Agents Actions 43(3-4), 149-156 (1994).
Kreutner, W., Chapman, R.W., Gulbenkian, A., et al. Antiallergic activity of loratadine, a non-sedating antihistamine. Allergy 42(1), 57-63 (1987).