Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: JNJ-7777120 is a potent and selective histamine H4 receptor antagonist, with a Ki value of approximately 4 nM against the human, mouse, and rat H4 receptors. Its Ki values for the histamine H1-3 receptors exceed 1 µM, regardless of species, and it has no or negligible effects on a range of other receptors and transporters. JNJ-777120 inhibits mast cell chemotaxis induced by 10 µM histamine (IC50 = 40 nM) and reduces neutrophil influx in mouse peritonitis models (10 mg/kg s.c.). It also impairs eosinophil and lymphocyte influx into airways during allergic airway inflammation.
JNJ-7777120 is supplied as a crystalline solid. A stock solution may be made by dissolving the JNJ-7777120 in the solvent of choice. JNJ-7777120 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of JNJ-7777120 in these solvents is approximately 2 mg/ml in ethanol and approximately 14 mg/ml in DMSO and DMF. JNJ-7777120 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, JNJ-7777120 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. JNJ-7777120 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Thurmond, R.L., Desai, P.J., Dunford, P.J., et al. A potent and selective histamine H4 receptor antagonist with antiinflammatory properties. J. Pharmacol. Exp. Ther. 309(1), 404-413 (2004).
Dunford, P.J., O’Donnell, N., Riley, J.P., et al. The histamine H4 receptor mediates allergic airway inflammation by regulating the activation of CD4+ T cells. J. Immunol. 176, 7062-7070 (2006).
Cowden, J.M., Riley, J.P., Ma, J.Y., et al. Histamine H4 receptor antagonism diminishes existing airway inflammation and dysfunction via modulation of Th2 cytokines. Respir. Res. 11(86), (2010).