Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The G protein-coupled receptors GPR109A and GPR109B are recognized to be receptors for hydroxy-carboxylic acid (HCA) metabolites, are abundant in adipocytes, and are relevant to atherosclerosis and dyslipidemia. IPBT-5CA is a selective agonist of GPR109B (HCA3; EC50 = 400 nM). It displays no activity at GPR109A (HCA2). IBC-293 inhibits forskolin-stimulated cAMP release in Chinese hamster ovary cells stably expressing GPR109B (EC50 = 54 nM) but not in cells expressing GPR109A. This is accompanied by a rapid and transient increase in intracellular calcium and activation of ERK1/2 through a pertussis toxin-sensitive Gi signaling pathway.
IPBT-5CA is supplied as a crystalline solid. A stock solution may be made by dissolving the IPBT-5CA in the solvent of choice. IPBT-5CA is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of IPBT-5CA in these solvents is approximately 5, 25, and 16 mg/ml, respectively. IPBT-5CA is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, IPBT-5CA should first be dissolved in DMF and then diluted with the aqueous buffer of choice. IPBT-5CA has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Ahmed, K., Tunaru, S., and Offermanns, S. Trends Pharmacol. Sci. 30(11), 557-562 (2009).