Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Peroxisome proliferator-activated receptor alpha (PPAR alpha) is a ligand-activated transcription factor found predominantly in the liver that is involved in the regulation of lipid homeostasis. Activation of PPAR alpha results in expression of a variety of genes, particularly those involved in fatty acid beta -oxidation, binding, and transport. GW 9578 is a potent agonist of PPAR alpha that activates the murine and human receptors with EC50 values of 0.005 and 0.05 µM, respectively. GW 9578 is highly selective for PPAR alpha compared to PPAR gamma and PPAR delta, which it activates in murine at EC50 values of 0.15 and 2.6 µM, respectively and in human at 1.0 and 1.4 µM, respectively. GW 9578 is a potent lipid lowering agent that may reduce insulin resistance. When 0.2 mg/kg GW 9578 was given orally once daily for three days, serum total LDL cholesterol was decreased 40-60% in male Sprague-Dawely rats. Obese Zucker rats treated with 5 mg/kg GW 9578 for nine days had markedly reduced serum insulin concentrations compared to controls.
GW 9578 is supplied as a solution in methyl acetate. To change the solvent, simply evaporate the methyl acetate under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as ethanol, DMSO, and dimethyl formamide (DMF) purged with an inert gas can be used. The solubility of GW 9578 in ethanol is approximately 2.5 mg/ml and approxmately 10 mg/ml in DMSO and DMF. GW 9578 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, the methyl acetate solution of GW 9578 should be diluted with the aqueous buffer of choice. GW 9578 has a solubility of approximately 0.3 mg/ml in a 1:2 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
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Mandard, S., Müller, M., and Kersten, S. Peroxisome proliferator-activated receptor a target genes. Cell. Mol. Life Sci. 61, 393-416 (2004).
Brown, P.J., Winegar, D.A., Plunket, K.D., et al. A ureido-thioisobutyric acid (GW9578) is a subtype-selective PPARa agonist with potent lipid-lowering activity. J. Med. Chem. 42, 3785-3788 (1999).
Guerre-Millo, M., Gervois, P., Raspé, E., et al. Peroxisome proliferator-activated receptor a activators improve insulin sensitivity and reduce adiposity. J. Biol. Chem. 275, 16638-16642 (2000).