Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR40 (free fatty acid receptor 1; FFAR1) and GPR120 (FFAR4) are G protein-coupled receptors (GPCR) that are activated by medium and long-chain fatty acids. In addition, there is evidence of a link between GPR40 and the ability of fatty acids to acutely potentiate insulin secretion. GW 9508 is a small-molecule agonist of GPR40 and GPR120. Stimulation of intracellular Ca2+ mobilization by GW 9508 in human embryonic kidney (HEK) 293 cells expressing GPR40 and GPR120 was observed with EC50 values of 47 nM and 2.2 µM, respectively. GW 9508 dose dependently potentiates glucose-stimulated insulin secretion and the KCl-mediated increase in insulin secretion in MIN6 cells.
GW 9508 is supplied as a crystalline solid. A stock solution may be made by dissolving the GW 9508 in an organic solvent purged with an inert gas. Solvents such as ethanol, DMSO, and dimethyl formamide purged with an inert gas can be used. The solubility of GW 9508 in these solvents is approximately 15 mg/ml. GW 9508 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, GW 9508 should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. GW 9508 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Briscoe, C.P., Peat, A.J., McKeown, S.C., et al. Pharmacological regulation of insulin secretion in MIN6 cells through the fatty acid receptor GPR40: Identification of agonist and antagonist small molecules. Br. J. Pharmacol. 148, 619-628 (2006).