Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The peripheral cannabinoid (CB2) receptor is a G protein-coupled receptor (GPCR) that is localized predominantly in immune cells, monocytes, macrophages, and in several peripheral organs and binds the active component of cannabis, Δ9-tetrahydrocannabinol, as well as anandamide, an endogenous CB receptor ligand. GW 842166X is a peripheral cannabinoid (CB2) receptor agonist with ED50 values of 91 and 63 nM in rat and human, respectively. When administered orally to rats in the Freund's complete adjuvant (FCA) model of inflammatory pain, GW 842166X is highly potent with an ED50 value of 0.1 mg/kg and full reversal of hyperalgesia at 0.3 mg/kg.
GW 842166X is supplied as a crystalline solid. A stock solution may be made by dissolving the GW 842166X in an organic solvent purged with an inert gas. GW 842166X is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of GW 842166X in ethanol is approximately 0.3 mg/ml and approximately 10 mg/ml in DMSO and DMF. GW 842166X is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, GW 842166X should first be dissolved in DMF and then diluted with the aqueous buffer of choice. GW 842166X has a solubility of approximately 0.25 mg/ml in a 1:3 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Khanolkar, A.D., Lu, D., Ibrahim, M., et al. Cannabilactones: A novel class of CB2 selective agonists with peripheral analgesic activity. J. Med. Chem. 50, 6493-6500 (2007).
Giblin, G.M.P., O’Shaughnessy, C.T., Naylor, A., et al. Discovery of 2-[(2,4-dichlorophenyl)amino]-N-[(tetrahydro- 2H-pyran-4-yl)methyl]-4-(trifluoromethyl)-5-pyrimidinecarboxamide, a selective CB2 receptor agonist for the treatment of inflammatory pain. J. Med. Chem. 50(11), 2597-2600 (2007).