Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR120 (free fatty acid receptor 4/FFAR4) is a G protein-coupled receptor expressed in intestine, adipocytes, and pro-inflammatory macrophages that is activated by long chain free fatty acids. It has been linked to the ability of fatty acids to produce anti-inflammatory effects and to acutely potentiate insulin secretion. GPR120 compound A is an orally available, high-affinity agonist of GPR120 (EC50 = ~0.35 µM) that demonstrates potent selectivity over another lipid-sensing G-protein, GPR40 (FFAR1). This compound exerts anti-inflammatory effects on macrophages in vitro and improves glucose tolerance, decreases hyperinsulinemia, increases insulin sensitivity, and decreases hepatic steatosis when included at 30 mg/kg body weight in a high-fat diet fed to obese mice.
GPR120 compound A is supplied as a crystalline solid. A stock solution may be made by dissolving the GPR120 compound A in the solvent of choice. GPR120 compound A is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of GPR120 compound A in these solvents is approximately 30 mg/ml. GPR120 compound A is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, GPR120 compound A should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. GPR120 compound A has a solubility of approximately 0.5 mg/ml in a 1:1 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Oh da, Y., Walenta, E., Akiyama, T.E., et al. A Gpr120-selective agonist improves insulin resistance and chronic inflammation in obese mice. Nat. Med. 20(8), 942-947 (2014).