Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR30 is a transmembrane G protein-coupled receptor (GPCR) localized to endoplasmic reticulum (ER) that binds estradiol with high affinity, activating multiple intracellular signaling pathways. G-1 is a nonsteroidal, high-affinity, selective agonist of GPR30 that binds with a Ki value of 11 nM. Competitive binding studies in endoplasmic reticulum alpha - (ER alpha -) and ER beta -expressing cells yielded Ki values for estradiol of 0.30 and 0.38 nM, respectively, with no substantial binding of G-1 at 1 µM. The discovery of G-1, a compound that does not bind classical ERs, should facilitate further physiological experiments to define the role of GPR30 in vivo.
G-1 is supplied as a crystalline solid. A stock solution may be made by dissolving the G-1 in an organic solvent purged with an inert gas. G-1 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of G-1 in these solvents is approximately 1, 20, and 30 mg/ml, respectively. G-1 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, G-1 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. G-1 has a solubility of approximately 0.15 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.