Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Sphingosine 1-phosphate (S1P) is an extracellular lipid mediator whose major effects are mediated through four of the S1P receptors (S1P1, S1P3, S1P4, and S1P5). S1P1 plays a key role in the immune system, regulating lymphocyte egress from lymphoid tissues into the circulation. S1P receptors have been shown to influence cell proliferation, morphology, and migration and are also expressed on a wide range of cells that are involved in many biological processes relevant to multiple sclerosis (MS). Fingolimod (FTY720 (free base) of Item No.) is a derivative of the fungal metabolite myriocin, a structural analog of sphingosine. In vivo, fingolimod is phosphorylated to form fingolimod-phosphate, which resembles naturally occurring S1P. Fingolimod-phosphate functions as an agonist at S1P1, S1P4, and S1P5 receptors (EC50 values of ~0.3-0.6 nM in vitro) and at 10-fold higher concentrations at S1P3 receptors (EC50 values of ~3 nM) but has no activity at S1P2 receptors. Fingolimod is a first-in-class orally bioavailable compound that has shown efficacy in advanced clinical trials for the treatment of MS through receptor-mediated actions both on the immune system and in the central nervous system.<a class="blurbReference" rel="#refer
Fingolimod is supplied as a crystalline solid. A stock solution may be made by dissolving the fingolimod in the solvent of choice. Fingolimod is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of fingolimod in these solvents is approximately 5, 10, and 20 mg/ml, respectively. Fingolimod is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline. If performing biological experiments, ensure the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
Huwiler, A., Kolter, T., Pfeilschifter, J., et al. Physiology and pathophysiology of sphingolipid metabolism and signaling. Biochim. Biophys. Acta 1485, 63-99 (2000).
Chun, J. and Hartung, H.P. Mechanism of action of oral fingolimod (FTY720) in multiple sclerosis. Clin. Neuropharmacol. 33(2), 91-101 (2010).
Brinkmann, V., Billich, A., Baumruker, T., et al. Fingolimod (FTY720): Discovery and development of an oral drug to treat multiple sclerosis. Nat. Rev. Drug Discov. 9(11), 883-897 (2010).