Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: DC260126 is a free fatty acid receptor 1 (FFAR1/GPR40) antagonist that inhibits GPR40-mediated Ca elevations stimulated by linoleic, oleic, palmitoleic, and lauric acid with IC50 values of 6.28, 5.96, 7.07, and 4.58 µM, respectively. At 10 mg/kg, it has been shown to inhibit glucose-stimulated insulin secretion, to reduce blood insulin levels, to improve insulin sensitivity, and to decrease the rate of pancreatic beta -cell apoptosis in obese diabetic db/db mice.
DC260126 is supplied as a crystalline solid. A stock solution may be made by dissolving the DC260126 in the solvent of choice. DC260126 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of DC260126 in these solvents is approximately 100, 20, and 30 mg/ml, respectively. DC260126 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, DC260126 should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. DC260126 has a solubility of approximately 0.25 mg/ml in a 1:3 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Hu, H., He, L., Gong, Z., et al. A novel class of antagonists for the FFAs receptor GPR40. Biochem. Bioph. Res. Commun. 390(3), 557-563 (2009).
Sun, T., Wang, T., Zhou, Y., et al. DC260126: A small-molecule antagonist of GPR40 that protects against pancreatic β-cells dysfunction in db/db mice. PLoS One 8(6), (2013).