Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Calcipotriol (hydrate) is a low-calcemic vitamin D receptor (VDR) agonist. Calcipotriol (hydrate) is about 200 times less potent in its effects on calcium metabolism than vitamin D (1,25[OH]D3). Binding of calcipotriol (hydrate) to the VDR increases AP-1, a transcription factor important for keratinocyte differentiation, and reduces expression of JunB protein, a transcriptional activator in the inflammatory response. Calcipotriol (hydrate) also induces expression of thymic stromal lymphopoietin, which triggers T-cell differentiation in keratinocytes.
Calcipotriol (hydrate) is supplied as a crystalline solid. A stock solution may be made by dissolving the calcipotriol (hydrate) in an organic solvent purged with an inert gas. Calcipotriol (hydrate) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of calcipotriol (hydrate) in these solvents is approximately 50 mg/ml. Calcipotriol (hydrate) is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, Calcipotriol (hydrate) should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. Calcipotriol (hydrate) has a solubility of approximately 0.15 mg/ml in a 1:5 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Kragballe, K. Vitamin D analogues in the treatment of psoriasis. J. Cell. Biochem. 49, 46-52 (1992).
Johansen, C., Kragballe, K., Rasmussen, M., et al. Activator protein 1 DNA binding activity is decreased in lesional psoriatic skin compared with nonlesional psoriatic skin. Br. J. Dermatol. 151, 600-607 (2004).
Li, M., Hener, P., Zhang, Z., et al. Topical vitamin D3 and low-calcemic analogs induce thymic stromal lymphopoietin in mouse keratinocytes and trigger an atopic dermatitis. Proc. Natl. Acad. Sci. USA 103(31), 11736-11741 (2006).