Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: AM580 is a retinoic acid receptor agonist that is selective for RAR alpha (Kd = 8 nM; AC50 = 0.36 nM) compared to RAR beta (Kd = 131 nM; AC50 = 24.6 nM) and RAR gamma (Kd = 450 nM; AC50 = 27.9 nM). It demonstrates greater specific binding to RAR alpha compared to retinoic acid, which exhibits little selectivity across RAR alpha, beta, or gamma . AM580 has been used in combination with the GSK3 beta inhibitor CHIR99021 to efficiently induce differentiation of human induced pluripotent stem cells into immediate mesoderm. It can also inhibit the proliferation of various tumor cells, inhibiting survival signaling pathways and inducing apoptosis.
AM580 is supplied as a crystalline solid. A stock solution may be made by dissolving the AM580 in the solvent of choice. AM580 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of AM580 in these solvents is approximately 10, 20, and 25 mg/ml, respectively. AM580 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, AM580 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. AM580 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.