Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The Ras/Raf-1 signalling pathway is a well-characterized system that links receptor tyrosine kinase (RTK) activation with changes in gene expression and cell behavior. Raf-1 is a serine/threonine protein kinase that phosphorylates and activates MAPK-kinase (MEK) in response to activation by Ras. ZM 336372 is a potent ATP-competitive inhibitor of Raf-1 in vitro (IC50 = 70 nM) with the paradoxical effect of inducing >100-fold activation of Raf-1 in whole cells. Activation of the Raf-1 signalling pathway using ZM 336372 in human carcinoid tumor cells results in induction of cell cycle inhibitors and suppression of cellular proliferation.
ZM 336372 is supplied as a crystalline solid. A stock solution may be made by dissolving the ZM 336372 in an organic solvent purged with an inert gas. ZM 336372 is soluble in organic solvents such as DMSO and dimethyl formamide. The solubility of ZM 336372 in these solvents is approximately 20 mg/ml. ZM 336372 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, ZM 336372 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. ZM 336372 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Hall-Jackson, C.A., Eyers, P.A., Cohen, P., et al. Paradoxical activation of Raf by a novel Raf inhibitor. Chemistry & Biology 6, 559-568 (1999).
Van Gompel, J.J., Kunnimalaiyaan, M., Holen, K., et al. ZM 336372, a Raf-1 activator, suppresses growth and neuroendocrine hormone levels in carcinoid tumor cells. Mol. Cancer Ther. 4(6), 910-917 (2005).