Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Vitamin D aids in the absorption of calcium and has central roles in bone formation and maintenance, hypertension, cancer and immunity. Vitamin D3 is an intermediate form of vitamin D which is obtained from dietary sources, such as fish, or through the conversion of 7-dehydrocholesterol by ultraviolet light. Vitamin D3 is subsequently metabolized to 25-hydroxyvitamin D3 and the active form 1,25-dihydroxyvitamin D3 by cytochrome P450 isoforms in the liver. 1,25-Dihydroxyvitamin D3 binds and activates the vitamin D receptor, which dimerizes with retinoid X receptor isoforms to alter gene expression.
Vitamin D3 is supplied as a crystalline solid. A stock solution may be made by dissolving the vitamin D3 in the solvent of choice. Vitamin D3 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of vitamin D3 in ethanol, DMSO, and DMF is approximately 30, 3, and 25 mg/ml, respectively. Vitamin D3 is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline. If performing biological experiments, ensure the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
Holick, M.F. Vitamin D deficiency. N. Engl. J. Med. 357(3), 266-281 (2007).
Peterlik, M., Boonen, S., Cross, H.S., et al. Vitamin D and calcium insufficiency-related chronic diseases: An emerging world-wide public health problem. Int. J. Environ. Res. Public Health 6, 2585-607 (2009).
Rosen, C.J. Vitamin D insufficiency. N. Engl. J. Med. 364(3), 248-54 (2011).
Houghton, L.A. and Vieth, R. The case against ergocalciferol (vitamin D2) as a vitamin supplement. Am. J. Clin. Nutr. 84, 694-697 (2006).
Kato, S., Kim, M.-S., Yamaoka, K., et al. Mechanisms of transcriptional repression by 1,25(OH)2 vitamin D. Curr. Opin. Nephrol. Hypertens. 16, 297-304 (2007).