Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Magnolol is a bioactive compound isolated from the bark of M. officinalis that has been used in Asian traditional medicine for the treatment of anxiety, sleep disorders, and allergic diseases. It can activate cannabinoid (CB) receptors, behaving as a partial agonist with selectivity for the peripheral CB2 subtype (EC50 = 3.28 µM; Ki = 1.44 µM). Tetrahydromagnolol is a major metabolite of magnolol that is 19-fold more potent than magnolol as a peripheral CB2 receptor agonist (EC50 = 0.17 µM; Ki = 0.42 µM). It also behaves as an antagonist at GPR55, a CB-related orphan receptor (KB = 13.3 µM).
Tetrahydromagnolol is supplied as a crystalline solid. A stock solution may be made by dissolving the tetrahydromagnolol in the solvent of choice. Tetrahydromagnolol is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of tetrahydromagnolol in ethanol and DMF is approximately 20 mg/ml and approximately 16 mg/ml in DMSO. Tetrahydromagnolol is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, tetrahydromagnolol should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. Tetrahydromagnolol has a solubility of approximately 0.16 mg/ml in a 1:5 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Rempel, V., Fuchs, A., Hinz, S., et al. Magnolia extract, magnolol, and metabolites: Activation of cannabinoid CB2 receptors and blockade of the related GPR55. ACS Med. Chem. Lett. 4, 41-45 (2013).