Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The free fatty acid receptor 1 (FFAR1; GPR40) is highly expressed in pancreatic beta -cells and activated by medium and long-chain fatty acids. There is evidence of a link between FFAR1 (GPR40) and the ability of fatty acids to amplify glucose-stimulated insulin secretion, making this signaling pathway a potential target for regulating diabetes, obesity, and other metabolic disorders. TAK-875 is a selective FFAR1 (GPR40) agonist (EC50 = 26 nM) that does not exhibit activity on the related FFARs FFAR2 (GPR43) or FFAR3 (GPR41).
TAK-875 is supplied as a crystalline solid. A stock solution may be made by dissolving the TAK-875 in the solvent of choice. TAK-875 is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of TAK-875 in these solvents is approximately 30 mg/ml. TAK-875 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, TAK-875 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. TAK-875 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Briscoe, C.P., Tadayyon, M., Andrews, J.L., et al. The orphan G protein-coupled receptor GPR40 is activated by medium and long chain fatty acids. J. Biol. Chem. 278(13), 11303-11311 (2003).
Itoh, Y., Kawamata, Y., Harada, M., et al. Free fatty acids regulate insulin secretion from pancreatic β cells through GPR40. Nature 422, 173-176 (2003).
Steneberg, P., Rubins, N., Bartoov-Shifman, R., et al. The FFA receptor GPR40 links hyperinsulinemia, hepatic steatosis, and impaired glucose homeostasis in mouse. Cell Metab. 1, 245-258 (2005).
Christiansen, E., Due-Hansen, M.E., Urban, C., et al. Free fatty acid receptor 1 (FFA1/GPR40) agonists: Mesylpropoxy appendage lowers lipophilicity and improves ADME properties. J. Med. Chem. 55, 6624-6628 (2012).