Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The peroxisome proliferator-activated receptor- gamma (PPAR gamma) is the nuclear receptor responsible for transducing the therapeutic activity of the thiazolidinediones (TZDs). TZDs are a group of structurally related synthetic PPAR gamma receptor agonists with antidiabetic actions in vivo. There are many PPAR gamma agonists, including 15-deoxy-Δ12,14-prostaglandin J2 and azelaoyl PAF, which are naturally derived. However, only a few antagonists have been reported. T0070907 is a potent and selective antagonist of the human PPAR gamma with an apparent IC50 of 1 nM for the binding inhibition of rosiglitazone, a reference TZD. T0070907 covalently binds to Cys313 of PPAR gamma, inducing conformational changes that block the recruitment of transcriptional cofactors to the PPAR gamma /RXR heterodimer.
T0070907 is supplied as a crystalline solid. A stock solution may be made by dissolving the T0070907 in an organic solvent purged with an inert gas. T0070907 is soluble in organic solvents such as DMSO and dimethyl formamide (DMF). The solubility of T0070907 in these solvents is approximately 10 mg/ml. T0070907 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, T0070907 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. T0070907 has a solubility of 0.2 mg/ml in a 1:4 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
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