Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Sulprostone is a metabolism resistant synthetic analog of PGE2. It is a selective agonist for EP3 receptors with a Ki value of 0.35 nM at the human recombinant EP3-III receptor and an IC50 of 0.01 µM for the inhibition of PGE2 binding. Sulprostone is a potent stimulator of uterine smooth muscle contractions with high abortifacient activity.
Sulprostone is supplied as a solution in methyl acetate. To change the solvent, simply evaporate the sulprostone under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as DMSO and dimethyl formamide purged with an inert gas can be used. The solubility of sulprostone in these solvents is approximately 10 mg/ ml. Sulprostone is soluble in ethanol at a concentration of approximately 25 mg/ml. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing any biological experiments. Avoid adding Sulprostoneto basic solutions (pH >7.4), as base treatment will degrade sulprostone to PGA and PGB compounds. Also, ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. If an organic solvent-free solution of sulprostone is needed, it can be prepared by evaporating the methyl acetate and directly dissolving the neat oil in aqueous buffers.The solubility of sulprostone in PBS (pH 7.2) is approximately 4 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Schaaf, T.K., Bindra, J.S., Eggler, J.F., et al. N-(Methanesulfonyl)-16-phenoxyprostaglandincarboxamides: Tissueselective uterine stimulants. J. Med. Chem. 24, 1353-1359 (1981).