Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: SU 5416 is a tyrosine kinase inhibitor best known as an inhibitor of the vascular endothelial growth factor receptor (VEGFR2; FLK1/KDR) and a suppressor of tumor vascularization, preventing the growth of multiple tumor types. In addition to inhibiting VEGFR2 (IC50 = 1 µM), SU 5416 also inhibits PDGFR (IC50 = 20 µM), c-Kit (IC50 = 30 nM), RET (IC50 = 170 nM), FLT3 (IC50 = 160 nM), ABL (IC50 = 11 µM), and ALK (IC50 = 1.2 µM). SU 5416 does not inhibit epidermal growth factor or fibroblast growth factor receptor tyrosine kinases (IC50 > 100 µM).
SU 5416 is supplied as a crystalline solid. A stock solution may be made by dissolving the SU 5416 in an organic solvent purged with an inert gas. SU 5416 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of SU 5416 in these solvents is approximately 0.25, 10, and 30 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. SU 5416 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, SU 5416 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. SU 5416 has a solubility of approximately 0.3 mg/ml in a 1:2 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Fong, T.A.T., Shawver, L.K., Sun, L., et al. Cancer Res 59, 99-106 (1999).
Litz, J., Warshamana-Greene, G.S., Sulanke, G. , et al. Lung Cancer 46, 283-291 (2004).
Mologni, L., Sala, E., Cazzaniga, S., et al. J. Mol. Endocrinol. 37, 199-212 (2006).