Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Tumor necrosis factor alpha (TNF alpha) is a primary mediator of many inflammatory conditions including rheumatoid arthritis, toxic shock, and sepsis. TNF alpha functions as a trimer and promotes receptor trimerization to activate proinflammatory and/or apoptotic signalling pathways. SPD-304 is an inhibitor of tumor necrosis factor alpha (TNF alpha). that prevents binding to the TNF Receptor 1 (TNFR1) with an IC50 of 22 µM. It binds to the biologically active TNF alpha trimer and promotes accelerated displacement of a single subunit to rapidly inactivate the cytokine. In a cell based assay, SPD-304 inhibited TNF alpha -mediated stimulation of IKB degradation with an IC50 of 4.6 µM.
SPD-304 is supplied as a crystalline solid. A stock solution may be made by dissolving the SPD-304 in an organic solvent purged with an inert gas. SPD-304 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of SPD-304 in these solvents is approximately 1 mg/ml in ethanol and DMF and approximately 10 mg/ml in DMSO. SPD-304 is sparingly soluble in aqueous buffers. If aqueous stock solutions are required for biological experiments, they can best be prepared by diluting the organic solvent into aqueous buffers or isotonic saline. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. We do not recommend storing the aqueous solution for more than one day.
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