Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Propenyl-L-NIO (hydrochloride) (ENIPO) is a potent, selective inhibitor of iNOS. The Ki values for inhibition of iNOS, nNOS, and eNOS by ENIPO are 17, 10.3 and 58.2 µM, respectively, as determined using initial rate binding kinetics. However, ENIPO is a time-dependent inhibitor that, with longer incubations, demonstrates reversible tight binding inhibition that is selective for iNOS over nNOS or eNOS. This selectivity results from iNOS exhibiting a 4-fold faster binding and 10-fold slower dissociation with ENIPO compared to nNOS. Ki values for ENIPO binding to iNOS and nNOS are 0.56 and 6 µM, respectively. Accurate determination of the Ki value of ENIPO for eNOS has not been determined but is expected to be 50-fold less than iNOS.
ENIPO (hydrochloride) is supplied as a crystalline solid. A stock solution may be made by dissolving the ENIPO (hydrochloride) in an organic solvent purged with an inert gas. ENIPO (hydrochloride) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of ENIPO (hydrochloride) in ethanol is approximately 10 mg/ml and approximately 2 mg/ml in DMSO and DMF. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of ENIPO (hydrochloride) can be prepared by directly dissolving the crystalline compound in aqueous buffers. The solubility of ENIPO (hydrochloride) in PBS, pH 7.2, is approximately 10 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Bretscher, L.E., Li, H., Poulos, T.L., et al. Structural characterization and kinetics of nitric-oxide synthase inhibition by novel N5-(iminoalkyl)- and N5-(iminoalkenyl)-ornithines. J. Biol. Chem. 278(47), 46789-46797 (2003).