Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase is the rate-limiting enzyme in the cholesterol biosynthetic pathway and the target of the statin class of cholesterol-lowering drugs. Pravastatin is a HMG-CoA reductase inhibitor that is a ring hydroxylated metabolite of mevastatin. It is a competitive inhibitor of HMG-CoA reductase with a Ki value of 2.3 nM for the active, open ring form of the molecule. Pravastatin, marketed as Pravachol™ or Lipostat™, is used to reduce LDL cholesterol and triglyceride levels and increase HDL cholesterol in the prevention of cardiovascular disease. In a study using dogs, five weeks of treatment with a dose 20 mg/kg per day reduced total plasma cholesterol levels by 29%.
Pravastatin is supplied as a crystalline solid. A stock solution may be made by dissolving the pravastatin in the solvent of choice. Pravastatin is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of pravastatin in these solvents is approximately 12.5, 20, and 25 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of pravastatin can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of pravastatin in PBS, pH 7.2, is approximately 10 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Tobert, J.A. Lovastatin and beyond: The history of the HMG-CoA reductase inhibitors. Nat. Rev. Drug Discov. 2, 517-526 (2003).
Corsini, A., Maggi, F.M., and Catapano, A.L. Pharmacology of competitive inhibitors of HMG-CoA reductase. Pharmacol. Res. 31(1), 9-27 (1995).