Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The cysteinyl leukotrienes (CysLT) LTC4 and LTD4 are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. Their biological effects are transduced by a pair of G protein-coupled receptors, CysLT1 and CysLT2. Pranlukast (ONO-1078) is a potent, selective and orally active cysteinyl leukotriene1 (CysLT1) receptor antagonist. Sold under the trade name Ultair, it was the first cysteinyl (peptidyl) leukotriene receptor antagonist (LTRA) marketed for the treatment of asthma. Clinical studies in Japan, Europe, and North America all show that pranlukast significantly attenuates bronchoconstriction in response to a variety of allergen challenges as well as to inhaled LTD4. Pranlukast binds to the human CysLT1 and CysLT2 receptors with IC50 values of approximately 4-7 nM and 3,600 nM, respectively.
Pranlukast is supplied as a crystalline solid. A stock solution may be made by dissolving the pranlukast in an organic solvent purged with an inert gas. Pranlukast is soluble in organic solvents such as DMSO and dimethyl formamide (DMF). The solubility of pranlukast in these solvents is approximately 10 and 20 mg/ml, respectively. Pranlukast is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, pranlukast should first be dissolved in DMF and then diluted with the aqueous buffer of choice. Pranlukast has a solubility of approximately 5 mg/ml in a 1:8 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.