Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Fatty acid amide hydrolase (FAAH) is the enzyme responsible for hydrolysis and inactivation of fatty acid amides including anandamide and oleamide. PF-750 is a potent, time-dependent, irreversible FAAH inhibitor with IC50 values of 0.6 and 0.016 µM when preincubated with recombinant human FAAH for 5 and 60 minutes, respectively. Activity-based profiling of various human and murine tissue proteome samples revealed that PF-750 is highly selective for FAAH relative to other serine hydrolases, showing no discernable off-site activity up to 500 µM.
PF-750 is supplied as a crystalline solid. A stock solution may be made by dissolving the PF-750 in an organic solvent purged with an inert gas. PF-750 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of PF-750 in ethanol is approximately 1 mg/ml and approximately 20 mg/ml in DMSO and DMF. PF-750 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, PF-750 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. PF-750 has a solubility of approximately 0.125 mg/ml in a 1:5 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Ahn, K., Johnson, D.S., Fitzgerald, L.R., et al. Novel mechanistic class of fatty acid amide hydrolase inhibitors with remarkable selectivity. Biochemistry 46, 13019-13030 (2007).