Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Fatty acid amide hydrolase (FAAH) is the enzyme responsible for hydrolysis and inactivation of fatty acid amides including anandamide and oleamide. PF-622 is a potent, time-dependent, irreversible FAAH inhibitor with IC50 values of 0.99 and 0.033 µM when preincubated with human recombinant FAAH for 5 and 60 minutes, respectively. Activity-based profiling of various human and murine tissue proteome samples revealed that PF-622 is highly selective for FAAH relative to other serine hydrolases, showing no discernable off-site activity up to 500 µM.
PF-622 is supplied as a crystalline solid. A stock solution may be made by dissolving the PF-622 in an organic solvent purged with an inert gas. PF-622 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of PF-622 in these solvents are approximately 0.3, 2, and 3 mg/ml, respectively. PF-622 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, PF-622 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. PF-622 has a solubility of approximately 0.15 mg/ml in a 1:5 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Ahn, K., Johnson, D.S., Fitzgerald, L.R., et al. Novel mechanistic class of fatty acid amide hydrolase inhibitors with remarkable selectivity. Biochemistry 46, 13019-13030 (2007).