Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Prostaglandin D2 (PGD2) is the major eicosanoid product of mast cells and is released in large quantities during allergic and asthmatic anaphylaxis. Two seven-transmembrane G protein-coupled receptors for PGD2, designated DP1 and CRTH2/DP2, have been identified. Activation of the DP1 receptor leads to an increase of intracellular cAMP levels. However, activation of DP2 results in mobilization of intracellular Ca2+ and an apparent decrease in intracellular cAMP. MK 0524 is a potent, selective DP1 receptor antagonist with Ki values of 0.57 nM and 0.75 µM for the DP1 and DP2 receptors, respectively. It inhibits PGD2-induced accumulation of cAMP in both washed platelets and platelet-rich plasma with IC50 values of 0.09 and 4.0 nM, respectively. In a sheep model of allergic rhinitis, 0.1 mg/kg MK 0524 completely blocked PGD2-induced nasal congestion. At a dose of 4 mg/kg, MK 0524 suppressed a nicotinic acid-induced vasodilatory response by 80% in a mouse model of flushing, an undesirable side-effect of niacin treatment for dyslipidemia
MK-0524 is supplied as a crystalline solid. A stock solution may be made by dissolving the MK-0524 in the solvent of choice. MK-0524 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of MK-0524 in ethanol is approximately 5 mg/ml and approximately 20 mg/ml in DMSO and DMF. MK-0524 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, MK-0524 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. MK-0524 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Sturino, C.F., O’Neill, G., Lachance, N., et al. Discovery of a potent and selective prostaglandin D2 receptor antagonist, [(3R)-4-(4-chloro-benzyl)-7-fluoro-5-(methylsulfonyl)-1,2,3,4-tetrahydrocyclopental[b]indol- 3-yl]-acetic acid. J. Med. Chem. 50, 794-806 (2007).
Cheng, K., Wu, T.-J., Wu, K.K., et al. Antagonism of the prostaglandin D2 receptor 1 suppresses nicotinic acid-induced vasodilation in mice and humans. Proc. Natl. Acad. Sci. USA 103(17), 6682-6687 (2006).
Marchetto, M.C.N., Muotri, A.R., Mu, Y., et al. Non-cell-autonomous effect of human SOD1G37R astrocytes on motor neurons derived from human embryonic stem cells. Cell. Stem Cell 3(6), 649-657 (2008).
Di Giorgio, F.P., Boulting, G.L., Bobrowicz, S., et al. Human embryonic stem cell-derived motor neurons are sensitive to the toxic effect of glial cells carrying an ALS-causing mutation. Cell. Stem Cell. 3(6), 637-648 (2008).