Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Peroxisome proliferator-activated receptors (PPARs) are activated by fatty acids and eicosanoids as well as antidyslipidemic agents. Among the receptor isotypes, PPAR alpha demonstrates a particular role in fatty acid oxidation whereas PPAR gamma is known to be involved in adipocyte differentiation and lipid storage. LT175 is a dual PPAR alpha / gamma ligand that displays partial agonism toward PPAR gamma (EC50s = 0.22, 0.26, and 0.48 µM for hPPAR alpha, mPPAR alpha, and hPPAR gamma, respectively). It exhibits low adipogenic activity in vitro and improves glucose homeostasis in diet-induced insulin resistant mice. LT175 was shown to disrupt the recruitment of coregulators, cyclic-AMP response element-binding protein-binding protein and nuclear corepressor 1, to PPAR gamma .
LT175 is supplied as a crystalline solid. A stock solution may be made by dissolving the LT175 in the solvent of choice. LT175 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of LT175 in these solvents is approximately 25, 50, and 30 mg/ml, respectively. LT175 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, LT175 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. LT175 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Gilardi, F., Giudici, M., Mitro, N., et al. LT175 is a novel PPARα/γ ligand with potent insulin-sensitizing effects and reduced adipogenic properties. J. Bio. Chem. 289(10), 6908-6920 (2014).