Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Sphingosine-1-phosphate (S1P) is a bioactive lipid that exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It exerts its activity by binding to five distinct G protein-coupled receptors, S1P1/EDG-1, S1P2/EDG-5, S1P3/EDG-3, S1P4/EDG-6, and S1P5/EDG-8. JTE-013 is a potent, selective sphingosine-1-phosphate 2 (S1P2) receptor antagonist that binds to the human and rat receptors with IC50 values of 17 and 22 nM, respectively, (IC50 values >10 µM for human S1P1 and S1P3). It reverses the inhibitory effects of S1P on cell migration of vascular endothelial cells and smooth muscle cells. Similarly, JTE-013 reverses the inhibition of S1P on invasion and migration of B16 melanoma cells. JTE-013 inhibits S1P-induced contraction of, as well as cyclic AMP accumulation in, coronary artery smooth muscle cells.
JTE-013 is supplied as a crystalline solid. A stock solution may be made by dissolving the JTE-013 in an organic solvent purged with an inert gas. JTE-013 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of JTE-013 in these solvents is approximately 20 mg/ml. JTE-013 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, JTE-013 should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. JTE-013 has a solubility of approximately 0.25 mg/ml in a 1:3 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Takuwa, Y., Takuwa, N., and Sugimoto, N. J. Biochem. 131, 767-771 (2002).
Ishii, I., Fukushima, N., Ye, X., et al. Annu. Rev. Biochem. 73, 321-354 (2004).
Kluk, M.J. and Hla, T. Biochim. Biophys. Acta 1582, 72-80 (2002).
Osada, M., Yatomi, Y., Ohmori, T., et al. Biochem. Biophys. Res. Commun. 299, 483-487 (2002).
Arikawa, K., Takuwa, N., Yamaguchi, H., et al. J. Biol. Chem. 278(35), 32841-32851 (2003).
Ohmori, T., Yatomi, Y., Osada, M., et al. Cardiovascular Res. 58, 170-177 (2003).