Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Peroxisome proliferator-activated receptor- alpha (PPAR alpha) is a ligand-activated transcription factor involved in the regulation of lipid homeostasis. Activation of PPAR alpha results in expression of a variety of genes, particularly those involved in fatty acid beta -oxidation, binding, and transport. GW 7647 is a potent, selective agonist of human and murine PPAR alpha It activates human PPAR alpha, PPAR gamma, and PPAR delta with EC50 values of 0.006, 1.1 and 6.2 µM, respectively, in a GAL4-PPAR binding assay. Similar EC50 values of 0.001, 1.3, and 2.9 were observed with the murine receptors. GW 7647 lowered triglycerides 93% and 60% in fat-fed hamsters and rats, respectively, at a dose of 3 mg/kg.
GW 7647 is supplied as a crystalline solid. A stock solution may be made by dissolving the GW 7647 in an organic solvent purged with an inert gas. GW 7647 is soluble in an organic solvent, such as DMSO. The solubility of GW 7647 in DMSO is approximately 15 mg/ml.
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Mandard, S., Müller, M., and Kersten, S. Peroxisome proliferator-activated receptor α target genes. Cell Mol. Life Sci. 61, 393-419 (2004).
Brown, P.J., Stuart, L.W., Hurley, K.P., et al. Identification of a subtype selective human PPARα agonist through parallel-array synthesis. Bioorganic & Medicinal Chemistry Letters 11, 1225-1227 (2001).