Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Diethylstilbestrol (DES) is a synthetic estrogen receptor agonist that was prescribed to pregnant women in the late 1930s. It was banned in 1971 because of possible links to increased risk of breast cancer in mothers along with congenital abnormalities and increased risk of cancer in offspring. DES is structurally related to, and is as potent as, estradiol in most assays, with a longer half-life. It has a relative binding affinity to sex hormone binding globulin (SHBG) of 0.2 compared to estradiol which has a relative binding affinity of 100. A concentration of 20 µM DES, displaces 30-13% of 3H-estradiol from SHBG in serum.
DES is supplied as a crystalline solid. A stock solution may be made by dissolving the DES in an organic solvent purged with an inert gas. DES is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of DES in these solvents is approximately 30 mg/ml. DES is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, DES should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. DES has a solubility of approximately 0.15 mg/ml in a 1:5 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Coelingh Bennink, H.J.T. Are all estrogens the same? Maturitas 47, 269-275 (2003).
Jury, H.H., Zaxharewski, T.R., and Hammond, G.L. Interactions between human plasma sex hormone-binding globulin and xenobiotic ligands. J. Steroid Biochem. Molec. Biol. 75, 167-176 (2000).