Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Combrestatin A4 (CA4) is a potent inhibitor of tubulin polymerization and displays strong inhibitory activity on tumor cell growth. Combrestatin A4 was shown to inhibit tumor growth in several cell lines including IMR32 (neuroblastoma), Hs746T (gastric carcinoma), CFPAC-1 (pancreatic carcinoma), and MCF-7 (breast cancer) with IC50 values of 2.16, 5.20, 3.46, and 18.47 nM, respectively. CA4 inhibits tubulin polymerization with an IC50 value of 2.2 µM.
CA4 is supplied as a crystalline solid. A stock solution may be made by dissolving the CA4 in an organic solvent purged with an inert gas. CA4 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of CA4 in DMSO is approximately 10 mg/ml and approximately 20 mg/ml in ethanol and DMF. CA4 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, CA4 should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. CA4 has a solubility of approximately 0.1 mg/ml in a 1:10 solution of ethanol:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Sun, L., Vasilevich, N.I., Fuselier, J.A., et al. Abilities of 3,4-diarylfuran-2-one analogs of combretastatin A-4 to inhibit both proliferation of tumor cell lines and growth of relevant tumors in nude mice. Anticancer Res. 24, 179-186 (2004).
Dey, P. Chromatin remodeling, cancer and chemotherapy. Current Medicinal Chemistry 13, 2909-2919 (2006).