Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Clemastine is a selective histamine H1 receptor antagonist (Ki = 0.26 nM) that also displays high affinity for muscarinic receptors (Ki = 16 nM). It has also recently been identified as a positive allosteric modulator of P2X7 receptor signaling. Clemastine has long been used to inhibit histamine-induced bronchoconstriction in asthma and airway hyperresponsive studies.
Clemastine (fumarate) is supplied as a crystalline solid. A stock solution may be made by dissolving the clemastine (fumarate) in the solvent of choice. Clemastine (fumarate) is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of clemastine (fumarate) in these solvents is approximately 0.1, 2.5, and 1.6 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of clemastine (fumarate) can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of clemastine (fumarate) in PBS, pH 7.2, is approximately 0.3 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Kubo, N., Shirakawa, S., Kuno, T., et al. Antimuscarinic effects of antihistamines: Quantitative evaluation by receptor-binding assay. Jpn. J. Pharmacol. 43(3), 277-282 (1987).
Nörenberg, W., Hempel, C., Urban, N., et al. Clemastine potentiates the human P2X7 receptor by sensitizing it to lower ATP concentrations. J. Biol. Chem. 286(13), 11067-11081 (2011).
Hartman, V., Magnussen, H., Holle, J.P., et al. Modulation of histamine-induced bronchoconstriction with inhaled, oral, and intravenous clemastine in normal and asthmatic subjects. Thorax 36(10), 737-740 (2013).