Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The sphingosine 1-phosphate (S1P) receptor S1P3 has roles in vasodilation, epithelial barrier function, cardioprotection, fibrosis, immunity, and inflammation. CYM 5541 is a selective, allosteric agonist of S1P3 (EC50 = 72-132 nM). It less effectively activates S1P1 (EC50 = 28-38 µM) and is ineffective at S1P2, S1P4, and S1P5 as well as a panel of other receptors, ion channels and transporters.
CYM 5541 is supplied as a crystalline solid. A stock solution may be made by dissolving the CYM 5541 in the solvent of choice. CYM 5541 is soluble in DMSO at a concentration of approximately 5 mg/ml.
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Rosen, H., Stevens, R.C., Hanson, M., et al. Sphingosine-1-phosphate and its receptors: Structure, signaling, and influence. Annu. Rev. Biochem. 82, 637-662 (2013).
Guerrero, M., Poddutoori, R., Pinacho-Crisostomo, F., et al. Probe development efforts for an allosteric agonist of the sphingosine 1-phosphate receptor 3 (S1P3), in Probe Reports from the NIH Molecular Libraries Program,1 (2011).
Jo, E., Bhhatarai, B., Repetto, E., et al. Novel selective allosteric and bitopic ligands for the S1P3 receptor. ACS Chem. Biol. 7(12), 1975-1983 (2012).