Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR35 is a G protein-coupled receptor that is activated by kynurenic acid and 2-acyl lysophosphatidic acids (e.g., 2-oleoyl lysophosphatidic acid). It is expressed predominantly on immune cells, the brain, and in the gastrointestinal tract. GPR35 is overexpressed in gastric cancer cells. CID-2745687 is a reversible, competitive antagonist of GPR35, blocking activation by the synthetic agonist pamoic acid with a Ki value of 12.8 nM. It less potently blocks activation of GPR35 by zaprinast (IC50 = 160 nM). It shows ~57-fold selectivity for GPR35 over the related receptor GPR55 (IC50 = 9.08 µM).
CID-2745687 is supplied as a crystalline solid. A stock solution may be made by dissolving the CID-2745687 in the solvent of choice. CID-2745687 is soluble in DMSO at a concentration of approximately 10 mg/ml.
Oka, S., Ota, R., Shima, M., et al. GPR35 is a novel lysophosphatidic acid receptor. Biochem. Biophys. Res. Commun. 395(2), 232-237 (2010).
Wang, J., Simonavicius, N., Wu, X., et al. Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35. J. Biol. Chem. 281(31), 22021-22028 (2006).
MacKenzie, A.E., Lappin, J.E., Taylor, D.L., et al. GPR35 as a novel therapeutic target. Front. Endocrinol. (Lausanne) 2, 68 (2011).
O’Dowd, B.F., Nguyen, T., Marchese, A., et al. Discovery of three novel G-protein-coupled receptor genes. Genomics 47, 310-313 (1998).
Okumura, S., Baba, H., Kumada, T., et al. Cloning of a G-protein-coupled receptor that shows an activity to transform NIH3T3 cells and is expressed in gastric cancer cells. Cancer Sci. 95(2), 131-135 (2004).
Heynen-Genel, S., Dahl, R., Shi, S., et al. Selective GPR35 antagonists: Antagonists for the orphan receptor GPR35, in Probe Reports from the NIH Molecular Libraries Program, Probe 3, 1 (2011).