Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: CGP 57380 is a selective inhibitor of MAP kinase-interacting kinase 1 (MNK1) in vitro (IC50 = 2.2 µM), with no inhibitory activity against p38, JNK1, ERK1/2, PKC, or Src-like kinases. It blocks the phosphorylation of eIF4E in response to TNF- alpha, arsenite, anisomycin, PMA, or fetal calf serum in 293 cells (IC50 = 3 µM). CGP 57380 also dose-dependently inhibits TNF- alpha production in RAW 264.7 cells that have been co-treated with agonists for Toll-like receptor (TLR)2 (HKLM), TLR4 (LPS), TLR6/2 (FSL), TLR7 (imiquimod), or TLR9 (CpG DNA), presumably by inhibiting MNK1.
CGP 57380 is supplied as a crystalline solid. A stock solution may be made by dissolving the CGP 57380 in an organic solvent purged with an inert gas. CGP 57380 is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of CGP 57380 in ethanol is approximately 0.25 mg/ml and approximately 30 mg/ml in DMSO and DMF. CGP 57380 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, CGP 57380 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. CGP 57380 has a solubility of approximately 0.5 mg/ml in a 1:1 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Knauf, U., Tschopp, C., and Gram, H. Negative regulation of protein translation by mitogen-activated protein kinaseinteracting kinases 1, Mol. Cell. Biol. 21(16), 5500-5511 (2001).
Rowlett, R.M., Chrestensen, C.A., Nyce, M., et al. MNK kinases regulate multiple TLR pathways and innate proinflammatory cytokines in macrophages. Am. J. Physiol. Gastrointest. Liver Physiol. 294, 452-459 (2008).