Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: The cysteinyl leukotrienes LTC4 and LTD4 are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. Their biological effects are transduced by a pair of G-protein coupled receptors, CysLT1 and CysLT2. BayCysLT2 is a potent CysLT2 receptor antagonist that inhibits radioligand binding of LTD4 to CysLT2 and CysLT1 receptor cell lines with IC50 values of 35 and >10,000 nM, respectively. BayCysLT2 concentration-dependently reverses LTC4-stimulated perfusion pressure increase and contractility decrease in isolated Langendorff-perfused guinea pig hearts.
BayCysLT2 is supplied as a crystalline solid. A stock solution may be made by dissolving the BayCysLT2 in the solvent of choice. BayCysLT2 is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of BayCysLT2 in these solvents is approximately 20 mg/ml. BayCysLT2 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, BayCysLT2 should first be dissolved in DMF and then diluted with the aqueous buffer of choice. BayCysLT2 has a solubility of approximately 0.1 mg/ml in a 1:9 solution of DMF:PBS (pH 7.2) using this method.We do not recommend storing the aqueous solution for more than one day.
Lynch, K.R., O’Neill, G.P., Liu, Q., et al. Characterization of the human cysteinyl leukotriene CysLT1 receptor. Nature 399, 789-793 (1999).
Heise, C.E., O’Dowd, B.F., Figueroa, D.J., et al. Characterization of the human cysteinyl leukotriene 2 receptor. J. Biol. Chem. 275, 30531-30536 (2000).
Sarau, H.M., Ames, R.S., Chambers, J., et al. Identification, molecular cloning, expression, and characterization of a cysteinyl leukotriene receptor. Mol. Pharmacol. 56, 657-663 (1999).
Harter, M., Erguden, J., Wunder, F., et al. Isophtalic acid derivatives. US 2006/0154912 A1 (2006), 1-104, 10/537,623.