Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR119, a glucose-dependent insulinotropic receptor, is a Gs protein-coupled receptor that is expressed in pancreas beta -cells and intestinal L-cells. Activation of GPR119 by the endogenous ligands lyso-phosphatidylcholine and oleoyl ethanolamide increases intracellular cAMP levels and promotes glucose-stimulated insulin secretion. AS-1269574 is an agonist of GPR119 that is effective both in isolated cells and in vivo. It increases cAMP levels in HEK293 cells transfected with human GPR119 (EC50 = 2.5 µM) and glucose-stimulated insulin secretion in mice (100 mg/kg).
AS-1269574 is supplied as a solution in ethanol. To change the solvent, simply evaporate the ethanol under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as DMSO and dimethyl formamide purged with an inert gas can be used. The solubility of AS-1269574 in these solvents is approximately 20 mg/ml. AS-1269574 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, the ethanolic solution of AS-1269574 should be diluted with the aqueous buffer of choice. AS-1269574 has a solubility of approximately 0.1 mg/ml in a 1:10 solution of DMF:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Overton, H.A., Babbs, A.J., Doel, S.M., et al. Deorphanization of a G protein-coupled receptor for oleoylethanolamide and its use in the discovery of small-molecule hypophagic agents. Cell Metab. 3, 167-175 (2006).
Soga, T., Ohishi, T., Matsui, T., et al. Lysophosphatidylcholine enhances glucose-dependent insulin secretion via an orphan G-protein-coupled receptor. Biochem. Biophys. Res. Commun. 326, 744-751 (2005).
Yoshida, S., Ohishi, T., Matsui, T., et al. Identification of a novel GPR119 agonist, AS1269574, with in vitro and in vivo glucose-stimulated insulin secretion. Biochem. Biophys. Res. Commun. 400, 437-441 (2010).