Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: Free fatty acid receptor 3 (FFAR3; GPR41) is a G protein-coupled receptor activated by short chain fatty acids (SCFAs). It is expressed in adipose tissue, the gastrointestinal tract, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation. AR420626 is a selective agonist of FFAR3 (GPR41; IC50 = 117 nM) that does not activate the related receptor FFAR2 (GPR43) at concentrations up to 100 µM. At 10 µM, it can stimulate a 1.26-fold increased release of glucagon-like peptide-1 from colonic crypt cultures.
AR420626 is supplied as a crystalline solid. A stock solution may be made by dissolving the AR420626 in the solvent of choice. AR420626 is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of AR420626 in these solvents is approximately 5 and 2 mg/ml, respectively. AR420626 is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, AR420626 should first be dissolved in DMSO and then diluted with the aqueous buffer of choice. AR420626 has a solubility of approximately 0.2 mg/ml in a 1:4 solution of DMSO:PBS (pH 7.2) using this method. We do not recommend storing the aqueous solution for more than one day.
Inoue, D., Tsujimoto, G., and Kimura, I. Regulation of energy homeostasis by GPR41. Front. Endocrinol. (Lausanne) 5, 1-3 (2014).
Engelstoft, M.S., Park, W., Sakata, I., et al. Seven transmembrane G protein-coupled receptor repertoire of gastric ghrelin cells. Mol. Metab. 2(4), 376-392 (2013).
Nøhr, M.K., Pedersen, M.H., Gille, A., et al. GPR41/FFAR3 and GPR43/FFAR2 as cosensors for short-chain fatty acids in enteroendocrine cells vs FFAR3 in enteric neurons and FFAR2 in enteric leukocytes. Endocrinology 154(10), 3552-3564 (2013).