Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Description: GPR183 (also known as Epstein-Barr virus-induced gene 2) is an orphan G protein-coupled receptor (GPCR) that is highly expressed in spleen and is required for humoral immune responses, including B cell migration and T cell-dependent antibody production. 7 alpha,25-dihydroxy Cholesterol (7 alpha,25-DHC) is a potent agonist of GPR183 that induces specific activation of the receptor with an EC50 value of 50 nM (Kd = 450 pM). At concentrations below 3 µM, it does not show any appreciable binding activity when tested against eight different nuclear hormone receptors including LXR and thirty-one different G protein-coupled receptors in a panel of reporter gene assays. 7 alpha,25-DHC can act as a potent chemokine, affecting migration of immune cells expressing GPR183 both in vitro (EC50 = 500 pM) and in vivo.
It should be stable for at least two years. 7α,25-DHC is supplied as a crystalline solid. A stock solution may be made by dissolving the 7α,25-DHC in the solvent of choice. 7α,25-DHC is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of 7α,25-DHC in DMSO is approximately 100 µg/ml and 2 and 20 mg/ml in ethanol and DMF, respectively. 7α,25-DHC is sparingly soluble in aqueous buffers. For maximum solubility in aqueous buffers, 7α,25-DHC should first be dissolved in ethanol and then diluted with the aqueous buffer of choice. 7α,25-DHC has a solubility of approximately 500 µg/ml in a 1:2 solution of ethanol:PBS (pH 7.2) using this method.We do not recommend storing the aqueous solution for more than one day.
Liu, C., Yang, X.V., Wu, J., et al. Oxysterols direct B-cell migration through EB12. Nature 475(7357), 519-523 (2011).
Hannedouche, S., Zhang, J., Yi, T., et al. Oxysterols direct immune cell migration via EBI2. Nature 475(7357), 524- 527 (2011).